Tehran says it ‘had nothing to do with’ attack near Syrian border after US president vows Washington will respond ‘at a time and in a manner of our choosing’

Hello and welcome to the Guardian’s live coverage of the crisis in the Middle East with me, Helen Livingstone.

Iran has denied any involvement in a drone strike that killed three US soldiers and injured dozens of others at a base in Jordan near the Syrian border.

The US will respond to the attack on its troops, Biden said. During a campaign event at South Carolina on Sunday following the strike, Biden said: “We shall respond.” In his earlier statement he also said: “Have no doubt – we will hold all those responsible to account at a time and in a manner of our choosing.”

Responsibility for Saturday’s attack on Tower 22, a military outpost on the Jordanian Syrian Iraqi borders was claimed by the Iranian-backed umbrella group Islamic Resistance. The groups have long been trying to drive the US troops out of Iraq and Syria, but have used the war in Gaza as the backdrop to intensify these efforts and broaden the battleground.

A senior official with Hamas, Sami Abu Zuhri, said the attacks on US forces were tied directly to Israel’s ongoing war in Gaza. Speaking to Reuters, Abu Zuhri said: “The killing of three American soldiers is a message to the US administration that unless the killing of innocents in Gaza stops, it must confront the entire nation.”

At least 165 Palestinians were killed and 290 injured over the past 24 hours, Gaza’s health ministry said on Sunday. That brings the total number of Palestinians killed in the Israeli onslaught on Gaza to 26,422 since 7 October, not including the thousands thought to be buried under the rubble of collapsed buildings. The huge death toll comes despite last week’s ICJ interim ruling that Israel must do everything possible to avoid killing Palestinian civilians.

Palestinian medics and residents said Israel continued to bomb areas around the two main hospitals in Khan Younis, hindering efforts by rescue teams to respond to desperate calls from people caught in the Israeli bombardment. “There is a complete failure of the healthcare system at Nasser and Al-Amal hospitals,” said health ministry spokesperson Ashraf al-Qidra.

The United Nations secretary general, António Guterres, has appealed to the 10 donor countries that have withdrawn funding from the UN Relief and Works Agency for Palestine Refugees to reconsider, saying the agency and Palestinians in desperate need should not be penalised due to the alleged acts of a dozen staff. Guterres said nine UNRWA staff had already been dismissed for alleged involvement in Hamas’s attack on Israel on 7 October and any UN employee involved in acts of terror would be held accountable, including through criminal prosecution. Aid agencies have repeatedly warned in recent weeks that ordinary Gazans are at risk of famine after Israel stopped most food aid from entering the territory and cut off water supplies.

Talks on Sunday initiated by Qatar, the US and Egypt and aimed at brokering a hostage deal between Israel and Hamas were “constructive” but “significant gaps” remain, a statement from the Israeli prime minister’s office has said. US officials have reportedly proposed an initial 30-day temporary ceasefire to allow for the remaining female, elderly and wounded Israeli hostages to be freed. This would be followed by a second 30-day pause where Israeli soldiers and male hostages would be released, in tandem with an increase in the trickle of aid permitted into Gaza.

Far-right Israeli ministers and ministers belonging to Benjamin Netanyahu’s Likud party have attended a conference on the resettlement of Gaza, at which the national security minister Itamar Ben Gvir said Israelis needed “to find a legal way to voluntarily emigrate [Palestinians],” Haaretz newspaper has reported. Communications minister Shlomo Karhi told the conference that in war, “‘voluntary’ is at times a state you impose [on someone] until they give their consent.’”

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