It’s the aquarium mystery that has gripped the world – just how did a ‘virgin stingray’ fall pregnant while living alone in a tank?

But after months of investigation – and a male shark falsely accused of sexual assault – it seems that mystery may finally have been solved. 

It’s been revealed that Charlotte the stingray at an aquarium in North Carolina has parthenogenesis – a rare type of asexual reproduction where offspring develop from unfertilised eggs.

Experts at the aquarium have now admitted it would have been ‘impossible’ for her to have mated with one of the five small sharks that share her tank. 

Now, questions will inevitably be asked of the zoo, which has enjoyed wall to wall publicity since the story first broke. 

Charlotte, who has spent much of her life at the Aquarium and Shark Lab in Hendersonville, North Carolina is expected to give birth to up to four pups in the next fortnight

Charlotte, who has spent much of her life at the Aquarium and Shark Lab in Hendersonville, North Carolina is expected to give birth to up to four pups in the next fortnight

Charlotte, who has spent much of her life at the Aquarium and Shark Lab in Hendersonville, North Carolina is expected to give birth to up to four pups in the next fortnight

A scientist said: 'We should set the record straight that there aren't some shark-ray shenanigans happening here'

A scientist said: 'We should set the record straight that there aren't some shark-ray shenanigans happening here'

A scientist said: ‘We should set the record straight that there aren’t some shark-ray shenanigans happening here’

What is parthenogenesis? 

Parthenogenesis (PG) is an asexual reproduction in which a female can produce an embryo without fertilizing an egg with sperm. 

In Greek, the name means the ‘virgin creation’.

Parthenogenesis can occur in some insects, fish, amphibians, birds and reptiles, but not mammals.

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This is despite the claim that an inter-species hook-up could have occurred between Charlotte and a shark earlier this month.  

Questions will inevitably be asked of the aquarium, which has enjoyed wall to wall publicity since the story first broke. 

Parthenogenesis can occur in some insects, fish, amphibians, birds and reptiles, but not mammals. 

Documented examples have included California condors, Komodo dragons and yellow-bellied water snakes.

Kady Lyons, a research scientist at the Georgia Aquarium in Atlanta, said Charlotte’s pregnancy is the only documented example she is aware of for round stingrays, although other kinds of sharks, skates and rays have had these kinds of pregnancies in human care.

‘I’m not surprised, because nature finds a way of having this happen,’ she said.

‘We don’t know why it happens. Just that it’s kind of this really neat phenomenon that they seem to be able to do.

‘We should set the record straight that there aren’t some shark-ray shenanigans happening here.’

Lab staff first thought Charlotte had a tumour when they noticed a lump on her back that was 'blowing up like a biscuit' before an ultrasound revealed the pregnancy

Lab staff first thought Charlotte had a tumour when they noticed a lump on her back that was 'blowing up like a biscuit' before an ultrasound revealed the pregnancy

Lab staff first thought Charlotte had a tumour when they noticed a lump on her back that was ‘blowing up like a biscuit’ before an ultrasound revealed the pregnancy

Aquarium staff had been doing ultrasound on our ray, Charlotte, since September, when she began to swell

Aquarium staff had been doing ultrasound on our ray, Charlotte, since September, when she began to swell

Aquarium staff had been doing ultrasound on our ray, Charlotte, since September, when she began to swell

Charlotte the stingray hasn’t shared a tank of water with a male of her species in at least eight years. 

She lives in a tank at Aquarium and Shark Lab in Hendersonville, North Carolina that’s about 2,200 gallons, about the the size of a construction dumpster.

But staff are hoping to get a tank nearly twice that size to accommodate Charlotte’s offspring. 

Brenda Ramer, executive director of the lab which encourages children to take an interest in science, said they may also install live cameras for an online livestream.

She said lab staff first thought Charlotte had a tumour in September when they noticed a lump on her back that was ‘blowing up like a biscuit’ before an ultrasound revealed the pregnancy.

Ms Ramer said: ‘We were all like, ‘Shut the back door. There’s no way’.

‘We thought we were overfeeding her. But we were overfeeding her because she has more mouths to feed.

‘It is very rare to happen. But it’s happening in the middle of the Blue Ridge Mountains in rural North Carolina, hundreds of miles from the ocean.’

Parthenogenesis is when eggs develop on their own without fertilization and create a clone of the mother. Pictured, staff at Aquarium and Shark Lab in Hendersonville, North Carolina

Parthenogenesis is when eggs develop on their own without fertilization and create a clone of the mother. Pictured, staff at Aquarium and Shark Lab in Hendersonville, North Carolina

Parthenogenesis is when eggs develop on their own without fertilization and create a clone of the mother. Pictured, staff at Aquarium and Shark Lab in Hendersonville, North Carolina

Round stingrays like Charlotte are abundant on the Pacific coasts of southern California and Mexico, often resting on the ocean’s sandy bottom near the shoreline.

In the wild they are typically the size of a small dinner plate and come in all shades of brown.

They eat small worms, crabs and mollusks, and they are preyed upon by certain types of sharks, seals and giant sea bass.

Just like other species of stingray, the round stingray is aptly named for the venomous serrated spine located on the tail. 

Although stingrays do not attack people, the tail spines can cause painful wounds if stepped upon or handled without caution.  

Monstrous stingrays up to 10 FEET long are tagged in the wild for the first time: National Geographic explorers are now tracking the wondrous world of the critically endangered species 

Approximately 11 monstrous stingrays measuring up to 10 feet long were tagged in the wild by divers, allowing them to see the wondrous world of the critically endangered species.

The mission revealed these elusive smalleye rays can dive more than 650 feet below the surface and swim hundreds of miles per day – facts not previously known to the scientific community.

Smalleye rays have only been previously studied through images, but the tagging is expected to produce new information that could lead to better protection for the species.

The program will take years to gather and analyze enough data to understand these creatures, but the National Geographic explorers who tagged the rays told NatGeo that it ‘promises a tantalizing glimpse into the lives of a mysterious species.

Read more 

This post first appeared on Dailymail.co.uk

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