As Australia faces an extinction crisis, some conservationists say that, to save some endangered species, we must have a national plan to freeze them

On 15 December 2014, inside a repurposed shipping container in the Atlanta Botanical Garden in the USA, Mark Mandica heard an unfamiliar animal call.

It was a reddish-brown frog about the size of a peach, called Toughie. He had lived silently for nine years since arriving from Panama – where the highly infectious chytrid fungus disease had arrived, leaving swathes of dead frogs in its wake.

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